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KMID : 0043320200430080821
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Archives of Pharmacal Research
2020 Volume.43 No. 8 p.821 ~ p.862
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Protective effects of L-theanine on rats with dextran sulfate sodium-induced inflammatory bowel disease
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Chen Ling
Xiao Wen-Jun
Yan Qiong-Xian
Gong Zhi-Hua
Zhang Sheng
Zeng Li
Yang Ming
Zhou Yan-He
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Abstract
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The aim of this study is to evaluate the anti-inflammatory and protective effects of L-theanine in inflammatory bowel disease (IBD) and to identify the underlying molecular mechanisms. Rats were pre-treated with L-theanine at 0, 50, 200, or 800 mg/kg/day. IBD was induced in rats using dextran sulfate sodium (DSS). Histopathological analysis suggests that L-theanine can suppress DSS-induced IBD with significant inhibition of inflammation in large and small intestinal tissues. Moreover, the 200 mg/kg/day L-theanine-treated DSS group had higher body and small intestine weights, a lower disease activity index and expression of inflammatory factors than the DSS group without pre-treatment. In RNA sequencing and tandem mass tag labeling analyses, large number of mRNAs and proteins expression level differed when compared with the DSS-induced rats with and without 200 mg/kg/day L-theanine pre-treatment. Moreover, Kyoto Encyclopedia of Genes and Genomes pathway analysis indicates the anti-inflammatory activities of L-theanine in DSS-induced IBD, with a high representation of genes in “Cholesterol metabolism” and “Retinol metabolism” pathways. Analysis of protein?protein interaction networks further indicates the involvement of these two pathways. These studies suggest that medium-dose L-theanine pre-treatment could ameliorate DSS-induced IBD through molecular mechanisms involving cholesterol and retinol metabolism.
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KEYWORD
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L-Theanine, Inflammatory bowel disease, Dextran sulfate sodium, Anti-inflammatory, Protective effects
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